Evaluating NDSU Breeding Material for Reaction to Anthracnose April 01, 2001
P.L Gross, K.F. Grafton, and R.S. Lamppa, Department of Plant Science and Plant Pathology, NDSU, Fargo, ND
Anthracnose ( Colletotrichum lindemuthianum ) has only been identified in a few instances in North Dakota, and with greater frequency in both Minnesota and the province of Manitoba, Canada. Anthracnose may have entered North Dakota recently on infected seed. Evaluation of the breeding material for resistance to anthracnose has been recently started.
Two isolates, races 7 and 73, of anthracnose were obtained from Michigan State University. The isolates were grown and maintained on Potato Dextrose Agar plates. Spores from race 7 were produced by growing the isolate on Bean Juice Agar for seven days. The spores were collected in sterile water from the agar surface and the concentration adjusted to 106 spores/ml. A group of regionally grown kidney, cranberry, pinto, and navy beans were grown in the greenhouse until the primary leaves were about 85% expanded. The underside of the primary leaves was inoculated with the spore suspension using an airbrush and the plants placed in moist chambers set at 100% humidity for 5 days. Plants were placed in the greenhouse and rated 10 days after inoculation. Rating was done by looking at percent vein infection. The first group of navy bean breeding materials was screened for anthracnose resistance the first week of March 2001.
Kidney beans had the highest percentage of anthracnose infection with cranberry beans next. Pinto beans and navy beans were found to have the least amount of infection. Fifteen navy bean lines were tested and only two lines showed consistent vein infection of 5-20%. Screening of plants will continue in March with race 7 and start with race 73.