Enhancement of Molecular Marker and Molecular Capabilities for Dry Bean Pathology
March 07, 2008
Rubella Goswami and Jack Rasmussen, Department of Plant Pathology, North Dakota State Univeristy
Molecular biology holds tremendous promise in providing the necessary tools for enhancing agricultural research. In addition its role in advancing breeding programs, these tools have been found to be instrumental in monitoring and diagnosing plant pathogens that affect crop production.
Objective: The purpose of this proposal was to request commodity money to purchase molecular biology laboratory equipment. This equipment would be used to conduct research on dry bean diseases in our region, including cooperation and collaboration with the dry bean breeding program.
Progress: The funds provided by the grower community along with support from NDSU have resulted in the initiation of a well equipped dry bean pathology laboratory in university. The equipment now available to members of the dry bean research team including pathologists and breeders comprise of a gradient PCR machine, gel electrophoresis units, DNA quantification apparatus, a mini centrifuge and other supplies. These are currently being used for direct pathogen identification from diseased tissue samples, study of pathogen variation and plant DNA analysis. Pictures showing members of the dry bean and pulse pathology group working with some of these equipments are included below:
Get electrophoreses for visualizing dry bean DNA
PCR for diagnosis of dry bean diseases